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CAPN2 Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

CAPN2 Antibody

貨號：

HYK-200113
規(guī)格：
圖片：
Western Blot
Positive WB detected in: 293T whole cell lysate
All lanes: CAPN2 antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 80, 72 kDa
Observed band size: 80 kDa

IHC image of CSB-PA004496LA01HU diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

產(chǎn)品詳情

產(chǎn)品描述：

CAPN2 polyclonal antibody CSB-PA004496LA01HU was produced in rabbit immunized by using the Recombinant Human Calpain-2 catalytic subunit protein (20-700AA) as the immunogen. The target protein CAPN2 is the large subunit of the ubiquitous enzyme, calpain 2, which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It proteolytically cleaves MYOC at 'Arg-226'. Diseases associated with CAPN2 include Nuclear Senile Cataract and Machado-Joseph Disease.
This Rabbit anti-Homo sapiens (Human) CAPN2 Polyclonal antibody was tested in the ELISA, WB, IHC, IF and IP applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the CAPN2 proteins of human-origin and may be used to detect the endogenous levels of CAPN2 protein.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) CAPN2 Polyclonal antibody
Uniprot No.：

P17655
基因名：

CAPN2
別名：

Calcium activated neutral proteinase 2 antibody; Calcium activated neutral proteinase antibody; Calcium-activated neutral protease 2; catalytic subunit antibody; Calcium-activated neutral proteinase 2 antibody; CALP80 antibody; Calpain 2 (m/II) large subunit antibody; Calpain 2 catalytic subunit antibody; Calpain 2 large catalytic subunit antibody; Calpain 2 large subunit antibody; Calpain 2; large [catalytic] subunit antibody; Calpain large polypeptide L2 antibody; Calpain M type antibody; Calpain M-type antibody; Calpain-2 catalytic subunit antibody; Calpain-2 large subunit antibody; Calpain2 antibody; CAN2_HUMAN antibody; CANP 2 antibody; CANP L2 antibody; CANP2 antibody; CANPL 2 antibody; CANPL2 antibody; CANPml antibody; Capa2 antibody; CAPN 2 antibody; CAPN2 antibody; FLJ39928 antibody; M calpain antibody; M calpin antibody; M type antibody; M-calpain antibody; mCANP antibody; Millimolar calpain antibody; Millimolar-calpain antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Calpain-2 catalytic subunit protein (20-700AA)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.