Western Blot Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate All lanes: AMBP antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
IHC image of CSB-PA001654YA01HU diluted at 1:150 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ELISA Protocol Western Blotting (WB) Protocol Immunohistochemistry (IHC) Protocol
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