Western Blot Positive WB detected in:Hela whole cell lysate,A549 whole cell lysate,Raw264.7 whole cell lysate,NIH/3T3 whole cell lysate,HepG2 whole cell lysate,Rat brain tissue,Rat heart tissue All lanes:Actin antibody at 0.95μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa
IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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