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Acetyl-Histone H2B type 1-B(K20)Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

Acetyl-Histone H2B type 1-B(K20)Antibody

貨號：

HYK-200284
規(guī)格：
圖片：
Western Blot
Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min
All lanes:Acetyl-Histone H2B type 1-B(K20)antibody at 0.135μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa

Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

產(chǎn)品詳情

Uniprot No.：

P33778
基因名：

HIST1H2BB
別名：

HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
反應(yīng)種屬：

Human, Mouse
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, ICC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:5000-1:10000
ICC	1:50-1:500
IF	1:30-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.